Coding
[TraGr]
Part:BBa_J23013:Design
Designed by: Will Bosworth Group: iGEM2006_Berkeley (2006-07-24)
TraGr open reading frame basic part
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1604
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 79
Illegal NgoMIV site found at 205
Illegal NgoMIV site found at 388
Illegal NgoMIV site found at 649
Illegal NgoMIV site found at 696
Illegal NgoMIV site found at 906
Illegal NgoMIV site found at 1402 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1710
Design Notes
JL10 Forward EcoRI oligo to bioBrick TraG: CCTAGGAATTCGCGGCCGCTTCTAGatgaagaaccgaaacaacgc JL11 Reverse SpeI oligo to bioBrick TraG: gacttACTAGTgaagcggctcatatcgtgatc
Source
PCR JL10/JL11 on Rlambda (?bp, EcoRI/SpeI, Neb2) Paste into pSB1A2-I13521 (EcoRI/SpeI, Neb2, Large 2062)