Coding
[TraGr]

Part:BBa_J23013:Design

Designed by: Will Bosworth   Group: iGEM2006_Berkeley   (2006-07-24)


TraGr open reading frame basic part


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1604
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 79
    Illegal NgoMIV site found at 205
    Illegal NgoMIV site found at 388
    Illegal NgoMIV site found at 649
    Illegal NgoMIV site found at 696
    Illegal NgoMIV site found at 906
    Illegal NgoMIV site found at 1402
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1710


Design Notes

JL10 Forward EcoRI oligo to bioBrick TraG: CCTAGGAATTCGCGGCCGCTTCTAGatgaagaaccgaaacaacgc JL11 Reverse SpeI oligo to bioBrick TraG: gacttACTAGTgaagcggctcatatcgtgatc


Source

PCR JL10/JL11 on Rlambda (?bp, EcoRI/SpeI, Neb2) Paste into pSB1A2-I13521 (EcoRI/SpeI, Neb2, Large 2062)

References